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Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure

Received: 6 June 2019     Accepted: 12 July 2019     Published: 26 July 2019
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Abstract

Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.

Published in Advances in Bioscience and Bioengineering (Volume 7, Issue 2)
DOI 10.11648/j.abb.20190702.13
Page(s) 27-31
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Soil, Microorganisms, Rhizosphere, Organic Manure

References
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[3] Ferando HC, Amanda V, Wright JS (1994). Tropical forest litter decomposition under seasonal drought nutrient release, fungi and bacteria. Oikos. (70): 183-190.
[4] Miyanoto T, Igaraslic T, Takahashi K (2002). Lignin–degradation ability of litter decomposing basidomycetes from picea forest of Hokkaida Myco. sci. (41): 105-110.
[5] Ibuot, A. A. and Bajhaiya, A (2013). Biodegradation of crude oil sludge using municipal solid waste as bulking agent. Asian Journal of Biological Sciences 6 (4) 207-213.
[6] Higga, T. (2001). Effect Microorganism: A biotechnology for mankind U.S. Department of Agriculture USA (16).
[7] Parr, J., Hornick, S. And Kaufmman, D. (2004) Use of microbial inoculants and organic fertilizers in Agricultural production Published by the food and fertilizer technology center Tapei 91 (32).
[8] Cheesbrough, M. (2006). District Laboratory practice in tropical countries (part 2) Cambridge University Press. Hon Kong. Pp 64-70.
[9] Bergey (1989). Bergey’s manual of systematic bacteriology Sterley JT (Ed). Vol. 3 Williams and Eilkins, Baltimore. p. 450.
[10] Nihorimbere, V., Ogena, M., Thonart, P. (2016). Beneficial effect of the Rhizosphere microbial community for plant growth and health. BASE 15: 2.
[11] Dundas, E., Paul, O., John, H. (2002). Mashavira is dated the Jains London ISBN 0-415.
[12] Amir, H., Pineau R., (1998). Influence of plant and cropping on microbiological characteristics of some new Caledonian ultamatic soils. Aust. J. Soil Res. 36, 3: 457-470.
[13] Okoh, L. A., Badejo, M. A., Nathaniel, I. T., Tian G. (1999). Studies on the bacteria, fungi, and springtails (Collembola) of an agroforestry arboretum in Nigeria. Pedobio 43: 18-27. Olivier, R., Bacchin, P., Robertiello, A., Oddo, N., Degen, L., And Tonolo, A., 2006. Microbial degradation of soil spills enhanced by a slow release fertilizer. App. Environ. Microbiol. 31: 629-634.
[14] O’ Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001). Plants and Fertilizers as drivers of change in microbial community structure and function in soil. Plant Soil (232): 135-45.
[15] Olivier, R., Bacchin, P., Robertiello, A., Oddo, N., Degen, L., And Tonolo, A., 2006. Microbial degradation of soil spills enhanced by a slow release fertilizer. App. Environ. Microbiol. 31: 629-634.
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  • APA Style

    Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. (2019). Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Advances in Bioscience and Bioengineering, 7(2), 27-31. https://doi.org/10.11648/j.abb.20190702.13

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    ACS Style

    Aniefon Alphonsus Ibuot; Iniobong Ime James; Mayen Godwin Ben; Christiana Utibe Etuk; Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv. BioSci. Bioeng. 2019, 7(2), 27-31. doi: 10.11648/j.abb.20190702.13

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    AMA Style

    Aniefon Alphonsus Ibuot, Iniobong Ime James, Mayen Godwin Ben, Christiana Utibe Etuk, Agnes Monday Jones, et al. Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure. Adv BioSci Bioeng. 2019;7(2):27-31. doi: 10.11648/j.abb.20190702.13

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  • @article{10.11648/j.abb.20190702.13,
      author = {Aniefon Alphonsus Ibuot and Iniobong Ime James and Mayen Godwin Ben and Christiana Utibe Etuk and Agnes Monday Jones and Emmanuel Anthony Umoren and Elizabeth Lazarus Akpan and Esther Ndarake Akpan},
      title = {Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {7},
      number = {2},
      pages = {27-31},
      doi = {10.11648/j.abb.20190702.13},
      url = {https://doi.org/10.11648/j.abb.20190702.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20190702.13},
      abstract = {Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.},
     year = {2019}
    }
    

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    T1  - Microbiological Analysis of Top Soil and Rhizosphere Treated with Organic Manure
    AU  - Aniefon Alphonsus Ibuot
    AU  - Iniobong Ime James
    AU  - Mayen Godwin Ben
    AU  - Christiana Utibe Etuk
    AU  - Agnes Monday Jones
    AU  - Emmanuel Anthony Umoren
    AU  - Elizabeth Lazarus Akpan
    AU  - Esther Ndarake Akpan
    Y1  - 2019/07/26
    PY  - 2019
    N1  - https://doi.org/10.11648/j.abb.20190702.13
    DO  - 10.11648/j.abb.20190702.13
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 27
    EP  - 31
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20190702.13
    AB  - Microbiological analysis of topsoil and rhizosphere treated with organic manure (Poultry droppings) was carried out. Soil samples were analyzed at three days interval (Day 1, Day 4, Day 7 and Day 10). The total bacterial count recorded for rhizosphere soil treated with poultry droppings (RS:P) had the highest bacterial count ranging between 2.1×106 and 5.7×106 CFU/g. Top soil treated with poultry droppings had total bacterial count ranging between 1.9×106 and 4.9×106 CFU/g. The controls (untreated rhizosphere soil) had a total bacterial count ranging between 2.6×106 and 4.0×106 CFU/g and untreated topsoil had bacterial count ranging between 1.4×106 and 2.4×106 CFU/g. Total fungal count for top soil treated with poultry droppings ranged between 0.2×106 and 0.9×106 CFU/g. Total fungal count for rhizosphere soil treated with poultry droppings ranged between 0.2×106 and 0.3×106 CFU/g. Untreated top soil had total fungal count ranging between 0.1×106 and 0.2×106 CFU/g while untreated rhizosphere soil had a total fungal count ranging between 0.1×106 and 0.2×106 CFU/g. Bacterial isolates identified with their percentage frequency of occurrence were Bacillus sp (16.8), Enterococus sp (8.4), Clostridium sp (4.0), Staphylococcus sp (8.0) Pseudomonas sp (15.6), Listeria sp (12.0), Micrococcus sp (14.0), Serratia sp (4.8) and Streptococcus sp (7.2). Fungal isolates identified with their percentage frequency of occurrence were Rhizosphere sp (26.7%), Penicillium sp (22.5%), Aspergillus sp (21.1%), Mucor sp (19.7%) and lastly Cladosporium sp (9.8%). Metabolites secreted by the root system act as chemical signal attracting high population of microorganisms. The application of organic manure to the soil enhanced the microbial population of the soil, hence the need to apply organic manure to soil to enhance agricultural sustainably.
    VL  - 7
    IS  - 2
    ER  - 

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Author Information
  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

  • Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

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