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Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens

Received: 15 April 2021     Accepted: 7 May 2021     Published: 20 May 2021
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Abstract

The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.

Published in Advances in Bioscience and Bioengineering (Volume 9, Issue 2)
DOI 10.11648/j.abb.20210902.11
Page(s) 20-24
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Antibiotic, Lactic Acid Bacteria, Pathogens, Multidrug Resistance, Animals

References
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[2] Dowlin, K., Nakasaki, K., Yanagisawa, M. and Kobayashi, K. (2017). Microbiological quality of fermented milk produced by repeated-batch culture. Journal of Bioscience andBioengineering, 105, 73-76.
[3] Dutta, T. K., Roychoudhury, P., Bandyopadhyay, S., and Chandra, R. (2011). “Detection and Characterization of Shiga Toxigenic Escherichia Coli from Piglets with or without Diarrhoea in Mizoram.” The Indian Journal of Animal Sciences 81 (9).
[4] Fraqueza, Y. (2015). Impact of small scale fermentation technology on food safety in developing countries. International Journal of Food Microbiology, 75, 213-229.
[5] Gad, E., Leroy, F. and De Vuyst, L. (2014). Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends in Food Science and Technology, 15, 67-78.
[6] Ghatak, S., Singha, A., Sen, A., Guha, C. Ahuja, A. Bhattacharjee, U. and Das, S. (2013). “Detection of New Delhi Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase Genes in Escherichia Coli Isolated from Mastitic Milk Samples.” Transboundary and Emerging Diseases 60 (5): 385–89.
[7] Hemlata, F., Fu, G. and Tuy, D., (2015). “Enumeration and Antibiotic Resistance Pattern of Staphylococcus Aureus from Raw Chicken Meat Sold in Bikaner City.” Journal of Pure and Applied Microbiology 9 (2).
[8] Jasmer, D. P., Goverse, A. and Smant, G. (2003). Parasitic nematode interactions with mammals and plants. Annual Review of Phytopathology. 41: 245-270.
[9] Kamal, M., Charton, J., Ballard, K., Latinovic, R. and Gulliford, M. (2015): Variations in antibiotic prescribing and consultation rates for acute respiratory infection in UK practices 1995-2000. British Journal of General Practice, 55: 603-608.
[10] Karamanis, E. M., Mathaiou, D. K., Moraitis, L. I., and Falagas, M. E. (2008): Fluoroquinolones versus beta-lactam base regimens for the treatment of osteomyelitis: a meta-analysis of randomized controlled trials. Spine, 33: E297-E304.
[11] Kumar, R., Yadav, B. R., Anand, S. K. and Singh, R. S. (2011). “Molecular Surveillance of Putative Virulence Factors and Antibiotic Resistance in Staphylococcus aureus Isolates Recovered from Intra-Mammary Infections of River Buffaloes.” Microbial Pathogenesis 51 (1-2): 31–38.
[12] Okaiyeto S. O., Salami O. S., Dnbirni S. A., Allam L. and Onoja, I. I. (2012). Clinical, Gross and Histopathological Changes Associated With Chronic Fasciolosis Infection in a Dairy Farm. Journal of veterinary advances, 2 (8): 444-448.
[13] Pan, D. and Yu, G. (2014). The relative effect of milk base, starter, and process on yogurt texture: A review. Critical Reviews in FoodScience and Nutrition, 44, 113-137.
[14] Singh, B. R., Jyoti, J., Chandra, M., Babu, N. and Sharma, G. (2009). “Drug Resistance Patterns of Salmonella Isolates of Equine Origin from India.” The Journal of Infection in Developing Countries 3 (02). 67.
[15] Tiwari, J. G., Chaudhary, S. P., Tiwari, H. K., Dutta, T. K., Saikia, P. and Hazarika, P. (2011). “Microbial Evaluation of Market Milk and Milk-Products of Mizoram, India with Special Reference to Staphylococcus Aureus.” The Indian Journal of Animal Sciences 81 (4).
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  • APA Style

    Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. (2021). Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Advances in Bioscience and Bioengineering, 9(2), 20-24. https://doi.org/10.11648/j.abb.20210902.11

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    ACS Style

    Abdulkadir Musliu; Sulaimon Adebisi; Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv. BioSci. Bioeng. 2021, 9(2), 20-24. doi: 10.11648/j.abb.20210902.11

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    AMA Style

    Abdulkadir Musliu, Sulaimon Adebisi, Samira Arzika. Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens. Adv BioSci Bioeng. 2021;9(2):20-24. doi: 10.11648/j.abb.20210902.11

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  • @article{10.11648/j.abb.20210902.11,
      author = {Abdulkadir Musliu and Sulaimon Adebisi and Samira Arzika},
      title = {Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens},
      journal = {Advances in Bioscience and Bioengineering},
      volume = {9},
      number = {2},
      pages = {20-24},
      doi = {10.11648/j.abb.20210902.11},
      url = {https://doi.org/10.11648/j.abb.20210902.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20210902.11},
      abstract = {The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Evaluation of Antibiotic Potential of Crude Culture Extract of Lactic Acid Bacteria on Multi-Drug Resistance Farm Animal Pathogens
    AU  - Abdulkadir Musliu
    AU  - Sulaimon Adebisi
    AU  - Samira Arzika
    Y1  - 2021/05/20
    PY  - 2021
    N1  - https://doi.org/10.11648/j.abb.20210902.11
    DO  - 10.11648/j.abb.20210902.11
    T2  - Advances in Bioscience and Bioengineering
    JF  - Advances in Bioscience and Bioengineering
    JO  - Advances in Bioscience and Bioengineering
    SP  - 20
    EP  - 24
    PB  - Science Publishing Group
    SN  - 2330-4162
    UR  - https://doi.org/10.11648/j.abb.20210902.11
    AB  - The present study evaluate the antibiotic potential of crude culture extract of lactic acid bacteria on multi-drugs resistance farm animal pathogen. Eight (8) different pathogens associated with Farm animals were used in this study. The pathogens were collected from different part of the body of different animal which include, Vagina of cow, Stool of cow; Male cow anus; Vagina of sheep; Vagina of goat, Male Goat Anus, Penis of goat and Female goat anus. The Confirmation of Pathogens, Isolation of crude extract of Lactic acid bacteria, Cell Free Supernatant and determination of antibacterial activity was done using standard method. The results show that Streptococcus agalactiae VIS, Staphylococcus aureus STC and Escherichia coli VRC were resistance to 4 out of 13 antibiotics. While Staphylococcus aureus FGA, and Escherichia coli (PEG, MGA and VRG) were resistances to 3 out of 13 antibiotic tested. Staphylococcus aureus MCA is sensitive to all antibiotics tested. Clear zone of inhibition was observed in both the Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria which ranged from 7mm to 25mm. The results further revealed that both Crude Culture Extract (CCE) and Cell Free Supernatant (CFS) of Lactic Acid Bacteria can be used in the in the treatment of multidrug resistance of pathogens. The isolation process of Crude Culture Extract (CCE) and it uses as an antibacterial agent is a simple process that do not required the use of any sophisticated equipment and techniques, thus it may be use in the treatment of multidrug resistance pathogens in both local and modern animal farms.
    VL  - 9
    IS  - 2
    ER  - 

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Author Information
  • Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria

  • Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria

  • Department of Science Technology, Waziri Umaru Federal Polytechnic, BirninKebbi, Nigeria

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