Pterocarpus macrocarpus, a perennial, woody tree, is considered as a commercially valuable species with various applications in furniture making, building materials, drug development, and dyeing. The genus Pterocarpus comprises 66 species growing throughout the tropics, with P. macrocarpus and P. indicus being morphologically closely related, leading to difficulties in distinguishing them via anatomical features. The calcium-dependent protein kinase (CPK) gene is a Ser/Thr protein kinase existing in plants, whose Ca2+ sensing and kinase activities play a significant role in plant growth, development and response to various stresses. However, current molecular methods such as DNA barcoding and phylogenetic analysis are time-consuming and labour-intensive. In the present study, a rapid and reliable real-time PCR method for wood identification of this species was established. Through target regions selection, primer/probe design and testing, method validation, specificity and sensitivity analysis, the most efficient real-time PCR approach taking partial CPK gene region as the target, was finally built up. It has also been proven to be highly specific and sensitive with a detection limit near 1.8 × 10-2 ng/μL. This study provides a useful tool for wood species discrimination for the proper utilization of this valuable timber, which will certainly benefit the wood industry towards a better and reasonable circumstance.
| Published in | Journal of Plant Sciences (Volume 11, Issue 4) |
| DOI | 10.11648/j.jps.20231104.12 |
| Page(s) | 121-127 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
High-Value Hardwood, Pterocarpus macrocarpus, Species Discrimination, Real-Time PCR
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APA Style
Jiaying Wang, Jihong Zhang, Weijun Duan, Xianfeng Chen, Yan Wang. (2023). Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR. Journal of Plant Sciences, 11(4), 121-127. https://doi.org/10.11648/j.jps.20231104.12
ACS Style
Jiaying Wang; Jihong Zhang; Weijun Duan; Xianfeng Chen; Yan Wang. Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR. J. Plant Sci. 2023, 11(4), 121-127. doi: 10.11648/j.jps.20231104.12
AMA Style
Jiaying Wang, Jihong Zhang, Weijun Duan, Xianfeng Chen, Yan Wang. Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR. J Plant Sci. 2023;11(4):121-127. doi: 10.11648/j.jps.20231104.12
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Download@article{10.11648/j.jps.20231104.12,
author = {Jiaying Wang and Jihong Zhang and Weijun Duan and Xianfeng Chen and Yan Wang},
title = {Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR},
journal = {Journal of Plant Sciences},
volume = {11},
number = {4},
pages = {121-127},
doi = {10.11648/j.jps.20231104.12},
url = {https://doi.org/10.11648/j.jps.20231104.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231104.12},
abstract = {Pterocarpus macrocarpus, a perennial, woody tree, is considered as a commercially valuable species with various applications in furniture making, building materials, drug development, and dyeing. The genus Pterocarpus comprises 66 species growing throughout the tropics, with P. macrocarpus and P. indicus being morphologically closely related, leading to difficulties in distinguishing them via anatomical features. The calcium-dependent protein kinase (CPK) gene is a Ser/Thr protein kinase existing in plants, whose Ca2+ sensing and kinase activities play a significant role in plant growth, development and response to various stresses. However, current molecular methods such as DNA barcoding and phylogenetic analysis are time-consuming and labour-intensive. In the present study, a rapid and reliable real-time PCR method for wood identification of this species was established. Through target regions selection, primer/probe design and testing, method validation, specificity and sensitivity analysis, the most efficient real-time PCR approach taking partial CPK gene region as the target, was finally built up. It has also been proven to be highly specific and sensitive with a detection limit near 1.8 × 10-2 ng/μL. This study provides a useful tool for wood species discrimination for the proper utilization of this valuable timber, which will certainly benefit the wood industry towards a better and reasonable circumstance.},
year = {2023}
}
TY - JOUR T1 - Rapid Species Discrimination of High Value Hardwood -- Pterocarpus macrocarpus via Real-Time PCR AU - Jiaying Wang AU - Jihong Zhang AU - Weijun Duan AU - Xianfeng Chen AU - Yan Wang Y1 - 2023/07/17 PY - 2023 N1 - https://doi.org/10.11648/j.jps.20231104.12 DO - 10.11648/j.jps.20231104.12 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 121 EP - 127 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20231104.12 AB - Pterocarpus macrocarpus, a perennial, woody tree, is considered as a commercially valuable species with various applications in furniture making, building materials, drug development, and dyeing. The genus Pterocarpus comprises 66 species growing throughout the tropics, with P. macrocarpus and P. indicus being morphologically closely related, leading to difficulties in distinguishing them via anatomical features. The calcium-dependent protein kinase (CPK) gene is a Ser/Thr protein kinase existing in plants, whose Ca2+ sensing and kinase activities play a significant role in plant growth, development and response to various stresses. However, current molecular methods such as DNA barcoding and phylogenetic analysis are time-consuming and labour-intensive. In the present study, a rapid and reliable real-time PCR method for wood identification of this species was established. Through target regions selection, primer/probe design and testing, method validation, specificity and sensitivity analysis, the most efficient real-time PCR approach taking partial CPK gene region as the target, was finally built up. It has also been proven to be highly specific and sensitive with a detection limit near 1.8 × 10-2 ng/μL. This study provides a useful tool for wood species discrimination for the proper utilization of this valuable timber, which will certainly benefit the wood industry towards a better and reasonable circumstance. VL - 11 IS - 4 ER -
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