Papaya cultivation is considered to be one of the most important fruit-growing activities in Cameroon, and plays a vital role in improving the livelihoods of local producers. However, leaf diseases caused by devastating fungi are a real obstacle to the development of this crop. The aim of this study was to evaluate the antifungal potential of Cymbopogon citratus essential oil against Alternaria alternata and Phomopsis carica-papayae, fungal agents associated with the deterioration of papaya leaves in the Moungo region of Cameroon. The essential oil of fresh C. citratus leaves was extracted by hydrodistillation. Analysis of the chemical composition by GC then GC/MS revealed Geranial (45.24%), Neral (35.57%) and Myrcene (7.21%) as the majority compounds. In vitro antifungal tests on mycelial growth by incorporation into agar gave MICs of 900 ppm and 700 ppm against A. alternata and P. carica-papayae respectively. The MIC of 700 ppm proved fungicidal on P. carica papayae, while for A. alternata the fungicidal effect was obtained at a concentration of 900 ppm. In situ, the essential oil proved significantly active in inhibiting necrosis caused by the conidial complex of the two pathogens on papaya leaves, with a percentage inhibition of 85.93% at a concentration of 6000 ppm. C. citratus EO could therefore be used as an alternative to chemical fungicides in the fight against papaya leaf disease pathogens.
| Published in | Journal of Plant Sciences (Volume 12, Issue 2) |
| DOI | 10.11648/j.jps.20241202.11 |
| Page(s) | 55-63 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Essential Oil, Cymbopogon citratus, Carica papaya L, Antifungal Activity, Leaf Diseases
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APA Style
Victor, D. M., Priscille, E., Steve, V., Bedine, A., Fabiola, D., et al. (2024). In vitro and in situ Activity of Cymbopogon citratus Essential Oil Against Alternaria alternata and Phomopsis carica-papayae, Causal Agents of Papaya Leaf Diseases. Journal of Plant Sciences, 12(2), 55-63. https://doi.org/10.11648/j.jps.20241202.11
ACS Style
Victor, D. M.; Priscille, E.; Steve, V.; Bedine, A.; Fabiola, D., et al. In vitro and in situ Activity of Cymbopogon citratus Essential Oil Against Alternaria alternata and Phomopsis carica-papayae, Causal Agents of Papaya Leaf Diseases. J. Plant Sci. 2024, 12(2), 55-63. doi: 10.11648/j.jps.20241202.11
AMA Style
Victor DM, Priscille E, Steve V, Bedine A, Fabiola D, et al. In vitro and in situ Activity of Cymbopogon citratus Essential Oil Against Alternaria alternata and Phomopsis carica-papayae, Causal Agents of Papaya Leaf Diseases. J Plant Sci. 2024;12(2):55-63. doi: 10.11648/j.jps.20241202.11
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Download@article{10.11648/j.jps.20241202.11,
author = {Davy Moussango Victor and Ebong Priscille and Voundi Steve and Ampère Bedine and Dikongue Fabiola and Ntah Moise and Youassi Olivier and Mekemzeu Patience and Tchameni Severin and Sameza Modeste},
title = {In vitro and in situ Activity of Cymbopogon citratus Essential Oil Against Alternaria alternata and Phomopsis carica-papayae, Causal Agents of Papaya Leaf Diseases},
journal = {Journal of Plant Sciences},
volume = {12},
number = {2},
pages = {55-63},
doi = {10.11648/j.jps.20241202.11},
url = {https://doi.org/10.11648/j.jps.20241202.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20241202.11},
abstract = {Papaya cultivation is considered to be one of the most important fruit-growing activities in Cameroon, and plays a vital role in improving the livelihoods of local producers. However, leaf diseases caused by devastating fungi are a real obstacle to the development of this crop. The aim of this study was to evaluate the antifungal potential of Cymbopogon citratus essential oil against Alternaria alternata and Phomopsis carica-papayae, fungal agents associated with the deterioration of papaya leaves in the Moungo region of Cameroon. The essential oil of fresh C. citratus leaves was extracted by hydrodistillation. Analysis of the chemical composition by GC then GC/MS revealed Geranial (45.24%), Neral (35.57%) and Myrcene (7.21%) as the majority compounds. In vitro antifungal tests on mycelial growth by incorporation into agar gave MICs of 900 ppm and 700 ppm against A. alternata and P. carica-papayae respectively. The MIC of 700 ppm proved fungicidal on P. carica papayae, while for A. alternata the fungicidal effect was obtained at a concentration of 900 ppm. In situ, the essential oil proved significantly active in inhibiting necrosis caused by the conidial complex of the two pathogens on papaya leaves, with a percentage inhibition of 85.93% at a concentration of 6000 ppm. C. citratus EO could therefore be used as an alternative to chemical fungicides in the fight against papaya leaf disease pathogens.
},
year = {2024}
}
TY - JOUR T1 - In vitro and in situ Activity of Cymbopogon citratus Essential Oil Against Alternaria alternata and Phomopsis carica-papayae, Causal Agents of Papaya Leaf Diseases AU - Davy Moussango Victor AU - Ebong Priscille AU - Voundi Steve AU - Ampère Bedine AU - Dikongue Fabiola AU - Ntah Moise AU - Youassi Olivier AU - Mekemzeu Patience AU - Tchameni Severin AU - Sameza Modeste Y1 - 2024/03/07 PY - 2024 N1 - https://doi.org/10.11648/j.jps.20241202.11 DO - 10.11648/j.jps.20241202.11 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 55 EP - 63 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.20241202.11 AB - Papaya cultivation is considered to be one of the most important fruit-growing activities in Cameroon, and plays a vital role in improving the livelihoods of local producers. However, leaf diseases caused by devastating fungi are a real obstacle to the development of this crop. The aim of this study was to evaluate the antifungal potential of Cymbopogon citratus essential oil against Alternaria alternata and Phomopsis carica-papayae, fungal agents associated with the deterioration of papaya leaves in the Moungo region of Cameroon. The essential oil of fresh C. citratus leaves was extracted by hydrodistillation. Analysis of the chemical composition by GC then GC/MS revealed Geranial (45.24%), Neral (35.57%) and Myrcene (7.21%) as the majority compounds. In vitro antifungal tests on mycelial growth by incorporation into agar gave MICs of 900 ppm and 700 ppm against A. alternata and P. carica-papayae respectively. The MIC of 700 ppm proved fungicidal on P. carica papayae, while for A. alternata the fungicidal effect was obtained at a concentration of 900 ppm. In situ, the essential oil proved significantly active in inhibiting necrosis caused by the conidial complex of the two pathogens on papaya leaves, with a percentage inhibition of 85.93% at a concentration of 6000 ppm. C. citratus EO could therefore be used as an alternative to chemical fungicides in the fight against papaya leaf disease pathogens. VL - 12 IS - 2 ER -
Laboratory of Biotechnology, University Institute of Technology, University of Douala, Douala, Cameroon; Department of Biochemistry, Faculty of Sciences, University of Douala, Douala, Cameroon
