Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya

Ebenezer Tatiekam Foka; Paul Martial Téné Tayo; Tatiana Flore Magni Pacha; Aristide Dzelamonyuy; Sylvain Leroy Kamdem Sado; Pierre Effa Onomo; Cécile Annie Ewané; Thaddée Boudjeko

doi:doi:10.11648/j.jps.20231106.11

Research Article |

Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya

Ebenezer Tatiekam Foka, Paul Martial Téné Tayo, Tatiana Flore Magni Pacha, Aristide Dzelamonyuy, Sylvain Leroy Kamdem Sado, Pierre Effa Onomo, Cécile Annie Ewané^*, Thaddée Boudjeko

Published in Journal of Plant Sciences (Volume 11, Issue 6)

Received: 14 September 2023 Accepted: 4 October 2023 Published: 9 November 2023

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Abstract

Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P <0.05) against plant pathogens. Apply optimize formulation at 1% on the detached cocoa leaves before inoculation with Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.

Published in	Journal of Plant Sciences (Volume 11, Issue 6)
DOI	10.11648/j.jps.20231106.11
Page(s)	171-181
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Theobroma cocoa L., Phytophthora megakarya, Bioformulation of Fungicide, Neem, Sida Weed, Oyster Shell

References

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Foka, E. T., Tayo, P. M. T., Pacha, T. F. M., Dzelamonyuy, A., Sado, S. L. K., et al. (2023). Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. Journal of Plant Sciences, 11(6), 171-181. https://doi.org/10.11648/j.jps.20231106.11

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ACS Style

Foka, E. T.; Tayo, P. M. T.; Pacha, T. F. M.; Dzelamonyuy, A.; Sado, S. L. K., et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J. Plant Sci. 2023, 11(6), 171-181. doi: 10.11648/j.jps.20231106.11

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AMA Style

Foka ET, Tayo PMT, Pacha TFM, Dzelamonyuy A, Sado SLK, et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J Plant Sci. 2023;11(6):171-181. doi: 10.11648/j.jps.20231106.11

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@article{10.11648/j.jps.20231106.11,
  author = {Ebenezer Tatiekam Foka and Paul Martial Téné Tayo and Tatiana Flore Magni Pacha and Aristide Dzelamonyuy and Sylvain Leroy Kamdem Sado and Pierre Effa Onomo and Cécile Annie Ewané and Thaddée Boudjeko},
  title = {Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {6},
  pages = {171-181},
  doi = {10.11648/j.jps.20231106.11},
  url = {https://doi.org/10.11648/j.jps.20231106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231106.11},
  abstract = {Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.
},
 year = {2023}
}

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TY  - JOUR
T1  - Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya
AU  - Ebenezer Tatiekam Foka
AU  - Paul Martial Téné Tayo
AU  - Tatiana Flore Magni Pacha
AU  - Aristide Dzelamonyuy
AU  - Sylvain Leroy Kamdem Sado
AU  - Pierre Effa Onomo
AU  - Cécile Annie Ewané
AU  - Thaddée Boudjeko
Y1  - 2023/11/09
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231106.11
DO  - 10.11648/j.jps.20231106.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 171
EP  - 181
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231106.11
AB  - Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.

VL  - 11
IS  - 6
ER  -

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Author Information

Ebenezer Tatiekam Foka

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Paul Martial Téné Tayo

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon

Contact Email
Tatiana Flore Magni Pacha

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Aristide Dzelamonyuy

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Biochemistry and Molecular Biology, Indiana University School of Medicine, Michigan, USA
Sylvain Leroy Kamdem Sado

Department of Microbiology, Faculty of Science, University of Yaounde I, Yaounde, Cameroon
Pierre Effa Onomo

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon
Cécile Annie Ewané

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon

Contact Email
Thaddée Boudjeko

Laboratory of Phytoprotection and Valorization of Genetic Resources, Biotechnology Centre-Nkolbisson, Yaounde, Cameroon; Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon

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APA Style

Foka, E. T., Tayo, P. M. T., Pacha, T. F. M., Dzelamonyuy, A., Sado, S. L. K., et al. (2023). Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. Journal of Plant Sciences, 11(6), 171-181. https://doi.org/10.11648/j.jps.20231106.11

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ACS Style

Foka, E. T.; Tayo, P. M. T.; Pacha, T. F. M.; Dzelamonyuy, A.; Sado, S. L. K., et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J. Plant Sci. 2023, 11(6), 171-181. doi: 10.11648/j.jps.20231106.11

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AMA Style

Foka ET, Tayo PMT, Pacha TFM, Dzelamonyuy A, Sado SLK, et al. Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya. J Plant Sci. 2023;11(6):171-181. doi: 10.11648/j.jps.20231106.11

Copy | Download

@article{10.11648/j.jps.20231106.11,
  author = {Ebenezer Tatiekam Foka and Paul Martial Téné Tayo and Tatiana Flore Magni Pacha and Aristide Dzelamonyuy and Sylvain Leroy Kamdem Sado and Pierre Effa Onomo and Cécile Annie Ewané and Thaddée Boudjeko},
  title = {Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya},
  journal = {Journal of Plant Sciences},
  volume = {11},
  number = {6},
  pages = {171-181},
  doi = {10.11648/j.jps.20231106.11},
  url = {https://doi.org/10.11648/j.jps.20231106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20231106.11},
  abstract = {Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.
},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Biofungicide Based Calcium, Azadirachta indica and Sida acuta Against Phytophthora megakarya
AU  - Ebenezer Tatiekam Foka
AU  - Paul Martial Téné Tayo
AU  - Tatiana Flore Magni Pacha
AU  - Aristide Dzelamonyuy
AU  - Sylvain Leroy Kamdem Sado
AU  - Pierre Effa Onomo
AU  - Cécile Annie Ewané
AU  - Thaddée Boudjeko
Y1  - 2023/11/09
PY  - 2023
N1  - https://doi.org/10.11648/j.jps.20231106.11
DO  - 10.11648/j.jps.20231106.11
T2  - Journal of Plant Sciences
JF  - Journal of Plant Sciences
JO  - Journal of Plant Sciences
SP  - 171
EP  - 181
PB  - Science Publishing Group
SN  - 2331-0731
UR  - https://doi.org/10.11648/j.jps.20231106.11
AB  - Plant pesticides are a complementary means to other control methods and an alternative to chemical control. The identification of potential plants that can be used in plant protection against pests is increasing enormously, but the availability of bioformulations is low. The new products developed for plant protection aim to protect them against a larger range of pathogens and include elicitors. The objective of this study is to produce an optimal emulsion bioformulation based on neem (Azadirachta indica A. Juss.), Sida (Sida acuta Burm. F.) and calcium, that can be used for plant protection against pests. The surface plot of the four factor simplex lattice designs data help to construct significant linear models for stability and activity. The best formulation was selected and optimize. The four Factor mixture designs and stability as well as activity models showed that the best formulation has neem oil (No) 12% (v/v), neem aqueous extract (Ne) 9% (w/v), sida weed hydroalcoholic extract (Se) 9% (w/v), Oyster shell’s calcium-rich extract (Ca) 6% (w/v) and tween-80 (Tw) 24% (v/v) with the production process “AQ + (Tw+No)”. Therefore, the optimize formulation has No 12% (v/v), Ne 9% (w/v), Se 9% (w/v), Ca 4.5%(w/v), calcium oxide (CaO 1.5% (w/v) and Tw 24% (v/v) with the production process “(Tw+No) + AQ”. The best formulation and the optimize one at ambient temperature have 100% of stability and a significant dose dependent activity (P Phytophthora megakarya reduces disease severity index from 4.2 to 1.5. These results suggest that our models and bioformulation can be useful for T. cocoa protection against P. megakarya, the causal agent of black pod disease.

VL  - 11
IS  - 6
ER  -

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